6OK3

Crystal structure of Sel1 repeat protein from Oxalobacter formigenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-21
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97940
Spacegroup name	C 1 2 1
Unit cell lengths	213.059, 44.976, 142.734
Unit cell angles	90.00, 92.84, 90.00

Refinement procedure

Resolution	47.519 - 2.353
R-factor	0.1928
Rwork	0.190
R-free	0.24750
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.390
High resolution limit [Å]	2.350	6.370	2.350
Rmerge	0.056	0.029	0.385
Rmeas	0.064	0.034	0.459
Rpim	0.030	0.016	0.245
Total number of observations	213350
Number of reflections	53054	2900	1614
<I/σ(I)>	9.4
Completeness [%]	93.0	95.7	57.9
Redundancy	4	4	3.1
CC(1/2)		0.994	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2M Lithium sulfate, Tris-HCl, 20% PEG4000