6OK0

Crystal structure of Sel1 repeat protein from Oxalobacter formigenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-28
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97924
Spacegroup name	P 1
Unit cell lengths	42.768, 68.587, 74.365
Unit cell angles	68.54, 79.25, 89.97

Refinement procedure

Resolution	39.629 - 2.174
R-factor	0.1899
Rwork	0.189
R-free	0.21530
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.210
High resolution limit [Å]	2.170	5.890	2.170
Rmerge	0.138	0.084	0.553
Rmeas	0.151	0.092	0.636
Rpim	0.061	0.036	0.303
Total number of observations	200503
Number of reflections	37770	1951	1575
<I/σ(I)>	7.2
Completeness [%]	93.3	95.8	78.6
Redundancy	5.3	6.2	3.1
CC(1/2)		0.984	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M magnesium chloride, 0.1M This-HCl, 20% PEG8000