6OJO
Dihydroneopterin aldolase (DHNA) from Yersinia pestis co-crystallized with pterine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 71.086, 81.489, 96.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.557 - 1.886 |
| R-factor | 0.1851 |
| Rwork | 0.184 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r2e |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.557 | 1.954 |
| High resolution limit [Å] | 1.886 | 1.886 |
| Rmeas | 0.110 | 0.584 |
| Number of reflections | 45556 | 4210 |
| <I/σ(I)> | 15.5 | 1.8 |
| Completeness [%] | 99.0 | 91.8 |
| Redundancy | 4.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 273 | 0.1 M BisTris pH 6.5 0.2 M CaCl2 0.05 M NaCl 47% MPD |
