6OJA

Crystal structure of the N. meningitides methionine-binding protein in its L-methionine bound conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	79.580, 87.650, 91.626
Unit cell angles	114.70, 104.41, 105.24

Refinement procedure

Resolution	34.201 - 1.550
R-factor	0.1632
Rwork	0.162
R-free	0.18140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.931
Data reduction software	TRUNCATE
Data scaling software	Aimless
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.201	34.201	1.590
High resolution limit [Å]	1.550	6.920	1.550
Rmerge	0.044	0.021	0.936
Rmeas	0.047	0.022	1.018
Number of reflections	277550	3187	20075
<I/σ(I)>	19.84	59.43	1.81
Completeness [%]	95.7	97.3	93.4
Redundancy	7.042	7.516	6.545
CC(1/2)	1.000	1.000	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	2.3M (NH4)2SO4, 0.1M sodium acetate pH 5