6OJ7

Respiratory syncytial virus fusion glycoprotein N-terminal heptad repeat domain+VIQKI I456F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127231
Spacegroup name	H 3
Unit cell lengths	32.285, 32.285, 203.841
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.960 - 1.450
R-factor	0.1802
Rwork	0.179
R-free	0.18870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ntx
RMSD bond length	0.013
RMSD bond angle	1.687
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.960	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.114	0.850
Rmeas	0.120	0.929
Rpim	0.037	0.366
Number of reflections	13875	1262
<I/σ(I)>	9.19	1.51
Completeness [%]	98.5	89.01
Redundancy	9.86	6.3
CC(1/2)	0.995	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	30 mM NaF, 30 mM NaBr, 30 mM NaI, 12.5% (v/v) 2-methyl-2,4-pentanediol, 12.5% (v/v) PEG 1000, 12.5% (w/v) PEG 3350 in 100 mM NaHEPES/MOPS buffer (pH 7.5)