6OJ2

Crystal structure of tRNA^ Ala(GGC) bound to the near-cognate 70S A-site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	211.000, 453.480, 625.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.986 - 3.200
R-factor	0.2382
Rwork	0.237
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.986	49.986	3.280
High resolution limit [Å]	3.200	14.310	3.200
Rmerge	0.246	0.075	2.199
Rmeas	0.261	0.080	2.334
Total number of observations	8581021
Number of reflections	971265	10898	71727
<I/σ(I)>	6.68	21.91	1.42
Completeness [%]	99.5	93.6	99.9
Redundancy	8.835	8.327	8.71
CC(1/2)	0.997	0.997	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP