6OIY
Structure of Escherichia coli bound to dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 192.176, 192.176, 299.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.870 - 3.290 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.399 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.380 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.252 | 0.790 |
| Number of reflections | 85237 | 5772 |
| <I/σ(I)> | 7.1 | 1.2 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 7.1 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1 M Tris-HCl pH 8.0 and 1.6 M Ammonium Sulfate |
