6OIY

Structure of Escherichia coli bound to dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 43 21 2
Unit cell lengths	192.176, 192.176, 299.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.870 - 3.290
R-factor	0.206
Rwork	0.205
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.399
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	3.380
High resolution limit [Å]	3.290	3.290
Rmerge	0.252	0.790
Number of reflections	85237	5772
<I/σ(I)>	7.1	1.2
Completeness [%]	99.0	98
Redundancy	7.1	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.1 M Tris-HCl pH 8.0 and 1.6 M Ammonium Sulfate