6OIX
Structure of Escherichia coli dGTPase bound to GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 191.587, 191.587, 292.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.750 - 3.150 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.337 | 3.020 |
| Rpim | 0.122 | 0.941 |
| Number of reflections | 87450 | 3646 |
| <I/σ(I)> | 7.5 | 0.9 |
| Completeness [%] | 99.8 | 54.3 |
| Redundancy | 6.4 | 2.2 |
| CC(1/2) | 0.998 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1 M Tris-HCl pH 8.0 and 1.6 M Ammonium Sulfate |
