6OIU

X-ray crystal structure of the ectodomain of the Toxoplasma gondii ME49 Aminopeptidase N (TGME49_224350)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9536
Spacegroup name	P 1 21 1
Unit cell lengths	92.930, 207.874, 102.867
Unit cell angles	90.00, 94.34, 90.00

Refinement procedure

Resolution	38.540 - 2.200
R-factor	0.193
Rwork	0.190
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ebg
RMSD bond length	0.001
RMSD bond angle	0.399
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.540	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	195835	19286
<I/σ(I)>	5.3	1.4
Completeness [%]	99.6	96
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	28 % PEG 3350, 0.1M Bis-Tris pH 5.0