6OIP

Crystal structure of MYST acetyltransferase domain in complex with inhibitor 34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	46.400, 55.920, 123.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.313 - 1.800
R-factor	0.1865
Rwork	0.185
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pq8
RMSD bond length	0.007
RMSD bond angle	0.977
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.313	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	30658	3054
<I/σ(I)>	13.4	4
Completeness [%]	99.9	100
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	20% PEG 3350, 0.2 M K/Na Tartrate