6OIE
The double PHD finger (DPF) of MORF in complex with histone H3K14cr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-03 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.583, 69.535, 70.187 |
| Unit cell angles | 90.00, 97.28, 90.00 |
Refinement procedure
| Resolution | 24.600 - 2.075 |
| R-factor | 0.1713 |
| Rwork | 0.166 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u2j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.120 |
| High resolution limit [Å] | 2.075 | 2.080 |
| Rmerge | 0.090 | 0.264 |
| Number of reflections | 18819 | 1810 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.1-1.3 M sodium citrate tribasic and 0.1 M sodium hepes |
