6OI7
Se-Met structure of apo- Escherichia coli dGTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 192.183, 192.183, 287.189 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.970 - 2.900 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.243 |
| Data reduction software | TRUNCATE |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.15.1_3469: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.277 | 3.080 |
| Number of reflections | 124944 | 4448 |
| <I/σ(I)> | 15.7 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.7 | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M Tris-HCl pH 8.0 and 1.6 M Ammonium Sulfate |
