6OHC
E. coli Guanine Deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.628, 80.588, 101.427 |
| Unit cell angles | 104.80, 105.72, 105.83 |
Refinement procedure
| Resolution | 48.910 - 2.300 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ood |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | |
| Rmeas | 0.097 | |
| Rpim | 0.039 | 0.504 |
| Number of reflections | 79427 | 4380 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 5.7 | 5.9 |
| CC(1/2) | 0.998 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Hepes pH 7.5 10% Peg 8000 |
