6OH9

Yeast Guanine Deaminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 2 2
Unit cell lengths	107.229, 107.229, 114.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.950 - 1.750
R-factor	0.142
Rwork	0.141
R-free	0.15590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ood
RMSD bond length	0.009
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.630	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.113	0.970
Number of reflections	67865	6573
<I/σ(I)>	14.2
Completeness [%]	99.8
Redundancy	8.8
CC(1/2)	0.998	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	17% Peg 3350 100 mM Hepes, pH 7.5 0.2 M ammonium sulfate