6OH8

Crystal structure of (E)-biformene synthase LrdC from Streptomyces sp. strain K155 in the dimeric form

Replaces:  5A0I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1000
Spacegroup name	P 1 21 1
Unit cell lengths	49.960, 104.160, 66.530
Unit cell angles	90.00, 111.25, 90.00

Refinement procedure

Resolution	52.080 - 2.170
R-factor	0.1974
Rwork	0.195
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LOW RESOLUTION SAD STRUCTURE
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.080	2.240
High resolution limit [Å]	2.170	2.170
Rmerge	0.152	1.426
Number of reflections	32973	2496
<I/σ(I)>	12	2.1
Completeness [%]	98.3	87.9
Redundancy	5.6	3.5
CC(1/2)	0.983	0.351

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		277	0.2 M MAGNESIUM ACETATE TETRAHYDRATE, 20% (W/V) PEG 3350