6OH8
Crystal structure of (E)-biformene synthase LrdC from Streptomyces sp. strain K155 in the dimeric form
Replaces: 5A0IExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.960, 104.160, 66.530 |
Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
Resolution | 52.080 - 2.170 |
R-factor | 0.1974 |
Rwork | 0.195 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | LOW RESOLUTION SAD STRUCTURE |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.080 | 2.240 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.152 | 1.426 |
Number of reflections | 32973 | 2496 |
<I/σ(I)> | 12 | 2.1 |
Completeness [%] | 98.3 | 87.9 |
Redundancy | 5.6 | 3.5 |
CC(1/2) | 0.983 | 0.351 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 277 | 0.2 M MAGNESIUM ACETATE TETRAHYDRATE, 20% (W/V) PEG 3350 |