6OH2
X-ray crystal structure of the mouse CMP-sialic acid transporter in complex with CMP, by lipidic cubic phase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.540, 49.530, 50.150 |
| Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
| Resolution | 47.000 - 2.580 |
| R-factor | 0.2415 |
| Rwork | 0.241 |
| R-free | 0.25194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.127 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.670 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Number of reflections | 10101 | 580 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 97.9 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293.15 | 26.7-30% PEG 300, 0.1 M MES pH 6.5, and 0.1 M NaCl |
