6OGX

Ternary complex of OX40R (TNFRSF4) bound to Fab1 and Fab2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.10505
Spacegroup name	I 2 2 2
Unit cell lengths	109.765, 125.249, 197.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.270 - 2.770
R-factor	0.205
Rwork	0.203
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.270	49.270	2.910
High resolution limit [Å]	2.770	9.190	2.770
Rmerge	0.092	0.024	0.853
Rmeas	0.099	0.026	0.923
Rpim	0.038	0.011	0.349
Number of reflections	41615	1037	4572
<I/σ(I)>	15.2
Completeness [%]	100.0	99.1	100
Redundancy	6.6	5.9	6.9
CC(1/2)	0.999	0.999	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris pH 8.5, 1.5 M D-L malic acid pH 7.0, 0.15 mM dimethylethylammonium propane sulfate (NDSB-195)