6OGB

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(3-I1Y), H148D; circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	51.810, 68.690, 61.640
Unit cell angles	90.00, 99.76, 90.00

Refinement procedure

Resolution	36.182 - 1.650
R-factor	0.157
Rwork	0.156
R-free	0.17760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zf3
RMSD bond length	0.007
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.182	1.709
High resolution limit [Å]	1.650	1.650
Rmerge	0.080	0.956
Rmeas	0.087	1.057
Rpim	0.036	0.443
Number of reflections	50444	4904
<I/σ(I)>	13.08	1.61
Completeness [%]	98.3	95.58
Redundancy	5.7	5.5
CC(1/2)	0.999	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	50 mM sodium acetate, pH 5.0, 100 mM NaCl, 7-8% PEG 3350