6OG9

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(3,5-F2Y), H148D; circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.127
Spacegroup name	P 1 21 1
Unit cell lengths	50.123, 70.131, 61.100
Unit cell angles	90.00, 96.31, 90.00

Refinement procedure

Resolution	36.594 - 1.798
R-factor	0.1698
Rwork	0.168
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zf3
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.594	1.863
High resolution limit [Å]	1.798	1.798
Rmerge	0.105	0.741
Rmeas	0.123	0.871
Rpim	0.064	0.453
Number of reflections	38844	3808
<I/σ(I)>	12.58	1.61
Completeness [%]	98.8	97.64
Redundancy	3.7	3.5
CC(1/2)	0.996	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	50 mM sodium acetate, pH 5.0, 100 mM NaCl, 25% PEG 3350