6OFP
Structure of the C-terminal cargo binding domain of human Bicaudal D2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.694, 36.958, 78.706 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 37.551 - 2.006 |
| R-factor | 0.2288 |
| Rwork | 0.226 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ytd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.667 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (dev_1819) |
| Refinement software | PHENIX (dev_1819) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 13628 | 542 |
| <I/σ(I)> | 14.9 | 2 |
| Completeness [%] | 98.0 | 85 |
| Redundancy | 3.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.05 | 293 | 0.1 M Tris, pH 8.05, 26% tert-Butanol, 40 mM CaCl2, 60 mM 1,6-Hexanediol |
