6OFO

Crystal structure of split green fluorescent protein (GFP); s10 circular permutant (194-195)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	50.749, 51.070, 97.298
Unit cell angles	90.00, 103.67, 90.00

Refinement procedure

Resolution	39.043 - 2.603
R-factor	0.2042
Rwork	0.203
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.005
RMSD bond angle	0.741
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.043	2.690
High resolution limit [Å]	2.600	2.600
Rmeas	0.192	1.790
Number of reflections	14682	1109
<I/σ(I)>	6.43	0.8
Completeness [%]	92.3	71.6
Redundancy	3.7	3.6
CC(1/2)	0.990	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M MES, pH 6.5, 20% MPD