6OF7
Crystal structure of the CRY1-PER2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 75 |
| Detector technology | PIXEL |
| Collection date | 2016-07-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.640, 81.230, 98.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.565 - 3.110 |
| R-factor | 0.2113 |
| Rwork | 0.208 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ct0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.428 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.565 | 3.280 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.170 | 0.650 |
| Number of reflections | 10939 | 1564 |
| <I/σ(I)> | 7.5 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 6.1 |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M magnesium chloride, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350 |
