6OE9

Crystal structure of p204 HIN1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	80
Detector technology	PIXEL
Collection date	2011-07-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	58.383, 58.383, 315.836
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.925 - 1.940
R-factor	0.1737
Rwork	0.172
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o0q
RMSD bond length	0.012
RMSD bond angle	1.325
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.920	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.082	0.334
Rmeas	0.084
Number of reflections	44585	2324
<I/σ(I)>	33.03	7.95
Completeness [%]	98.5	95.95
Redundancy	21.9	19.2
CC(1/2)	0.985	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.33 M ammonium sulfate, 0.05 M sodium acetate pH 4.5, and 0.2 M NaCl