6OE6

Structure of a Uncharacterized protein from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni (strain Fiocruz L1-130)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-07
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 31
Unit cell lengths	50.630, 50.630, 99.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.122 - 1.700
R-factor	0.1775
Rwork	0.176
R-free	0.20790
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.15rc1_3423))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.122	40.122	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.038	0.037	0.485
Rmeas	0.043	0.043	0.547
Total number of observations	150310
Number of reflections	31388	343	2372
<I/σ(I)>	22.21	43.6	2.91
Completeness [%]	99.9	94.5	99.9
Redundancy	4.789	4.385	4.706
CC(1/2)	0.999	0.997	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	Microlytic MCSG1 screen F8: 3500mM sodium formate: LpinA.19858.a.B2.PS38529 at 23.59mg/ml: cryo: direct: tray 306183 F8: puck lbo4-3. For experimental phasing, a crystal from the same well was incubated for 10sec in a solution of 90% reservoir and 10% 2.5M Sodium iodide in ethylene glycol and vitrified: tray: 306143 F8: puck: kzx0-14
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	Microlytic MCSG1 screen F8: 3500mM sodium formate: LpinA.19858.a.B2.PS38529 at 23.59mg/ml: cryo: direct: tray 306183 F8: puck lbo4-3. For experimental phasing, a crystal from the same well was incubated for 10sec in a solution of 90% reservoir and 10% 2.5M Sodium iodide in ethylene glycol and vitrified: tray: 306143 F8: puck: kzx0-14