6OD9
Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE MFX |
| Synchrotron site | SLAC LCLS |
| Beamline | MFX |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-03-20 |
| Detector | CS-PAD XPP |
| Wavelength(s) | 1.303 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.740, 92.740, 120.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.191 - 4.102 |
| R-factor | 0.2603 |
| Rwork | 0.258 |
| R-free | 0.30790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5btp |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.394 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 4.250 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Number of reflections | 9078 | 492 |
| <I/σ(I)> | 3.5 | 2 |
| Completeness [%] | 99.1 | 97.8 |
| Redundancy | 10 | 7.1 |
| CC(1/2) | 0.430 | 0.160 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 28-32% PEG 4000, 0.2 M lithium sulfate, 0.1 M Bis-Tris-HCl, pH 7 |
