6OD0
Structure of human CIB1 in complex with peptide inhibitor UNC10245092
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.858, 85.966, 67.287 |
| Unit cell angles | 90.00, 91.91, 90.00 |
Refinement procedure
| Resolution | 21.810 - 2.150 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xo5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.830 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 20253 | 1580 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 94.2 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 294 | 137.5 mM calcium acetate, 16 % PEG 3350, 20 mM Bis-Tris propane (pH 6.8) |
