6OCT
Crystal structure of human KCTD16 T1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.993, 68.993, 261.559 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.910 - 2.800 |
| R-factor | 0.2278 |
| Rwork | 0.227 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ocr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.780 | 3.130 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.118 | 0.740 |
| Rmeas | 0.131 | 0.831 |
| Rpim | 0.057 | 0.375 |
| Total number of observations | 181942 | |
| Number of reflections | 34280 | 9764 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 5.3 | 4.8 |
| CC(1/2) | 0.996 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 293.15 | 6% PEG 6000 2% glycerol 0.1 M sodium cacodylate trihydrate pH 6.0 |
