6OCC
JAK2 JH2 in complex with JAK190
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-22 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.660, 57.595, 61.662 |
| Unit cell angles | 90.00, 111.11, 90.00 |
Refinement procedure
| Resolution | 33.757 - 2.030 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5usz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmeas | 0.139 | 0.611 |
| Rpim | 0.056 | 0.298 |
| Number of reflections | 18725 | 855 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.6 | 94.6 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1M TRIS, PH 8.0 0.2M SODIUM ACETATE, 0.001 M TCEP, 12-24% PEG 4,000 |
