6OBN

The crystal structure of coexpressed SDS22:PP1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 65 2 2
Unit cell lengths	94.147, 94.147, 545.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.870 - 2.700
R-factor	0.2044
Rwork	0.202
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4MOV & 6HKW
RMSD bond length	0.006
RMSD bond angle	0.771
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.870	2.810
High resolution limit [Å]	2.700	2.700
Rmerge	0.203	1.623
Number of reflections	40847	4336
<I/σ(I)>	9
Completeness [%]	99.5
Redundancy	8.5
CC(1/2)	0.997	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	289	14% PEG4000, 6% MPD, sodium/potassium phosphate