6OBG

Ricin A chain bound to VHH antibody V8E6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	59.795, 77.885, 92.597
Unit cell angles	90.00, 98.95, 90.00

Refinement procedure

Resolution	45.735 - 1.996
R-factor	0.1905
Rwork	0.189
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	1.996	5.430	2.000
Rmerge	0.081	0.057	0.710
Rmeas	0.097	0.067	0.865
Rpim	0.052	0.036	0.487
Number of reflections	54931	2816	2570
<I/σ(I)>	5.3
Completeness [%]	96.2	95.7	91.8
Redundancy	3.2	3.3	2.9
CC(1/2)		0.995	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	295	100 mM MES (pH 6.0), 170 mM zinc acetate, 6% PEG 8,000, and 0.5% ethyl acetate