6OBF

JAK2 JH2 in complex with JAK179

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	44.246, 57.395, 60.617
Unit cell angles	90.00, 111.20, 90.00

Refinement procedure

Resolution	41.252 - 1.710
R-factor	0.1987
Rwork	0.198
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5usz
RMSD bond length	0.006
RMSD bond angle	0.758
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.710	1.710
Rmeas	0.101
Rpim	0.039	0.410
Number of reflections	31289	1569
<I/σ(I)>	18.6
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)		0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277.15	0.1M TRIS, PH 8.0 0.2M SODIUM ACETATE, 0.001 M TCEP, 12-24% PEG 4,000