6OAT

Structure of the Ganjam virus OTU bound to sheep ISG15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 18-ID
Synchrotron site	APS
Beamline	18-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	55.388, 55.388, 497.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.440 - 3.170
R-factor	0.2562
Rwork	0.251
R-free	0.30420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6oar
RMSD bond length	0.003
RMSD bond angle	0.610
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.150	3.150
Rpim	0.074
Number of reflections	12288	1139
<I/σ(I)>	11.2	1.1
Completeness [%]	85.0
Redundancy	6.7
CC(1/2)	0.994	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M MES pH 5.5, 15% PEG 20,000