6OAP
Crystal structure of a dual sensor histidine kinase in the green-light absorbing Pg state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.532, 72.532, 254.734 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.000 |
High resolution limit [Å] | 1.970 | 5.350 | 1.970 |
Rmerge | 0.071 | 0.038 | 1.139 |
Rmeas | 0.074 | 0.039 | 1.197 |
Rpim | 0.020 | 0.011 | 0.360 |
Number of reflections | 47992 | 2721 | 2336 |
<I/σ(I)> | 6 | ||
Completeness [%] | 97.2 | 97.8 | 97.2 |
Redundancy | 13 | 12.9 | 10.7 |
CC(1/2) | 0.999 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3350, magnesium chloride, HEPES. Crystals grown in dark |