6OAN
Structure of DBP in complex with human neutralizing antibody 053054
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 93 |
| Detector technology | CMOS |
| Collection date | 2016-08-09 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.480, 53.950, 142.780 |
| Unit cell angles | 90.00, 113.74, 90.00 |
Refinement procedure
| Resolution | 19.596 - 2.900 |
| R-factor | 0.2308 |
| Rwork | 0.230 |
| R-free | 0.25160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nuv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.542 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.079 | 0.720 |
| Number of reflections | 28614 | 2705 |
| <I/σ(I)> | 14.46 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 290 | 0.2M ammonium sulfate, 0.1 M sodium citrate pH 5.6, 25% w/v PEG 4000 |
