6OAF

Sudan virus nucleoprotein core domain in complex with VP35 chaperoning peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.2819
Spacegroup name	C 1 2 1
Unit cell lengths	126.840, 33.750, 101.860
Unit cell angles	90.00, 101.46, 90.00

Refinement procedure

Resolution	43.350 - 2.200
R-factor	0.2136
Rwork	0.212
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ztg
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.084	1.361
Rpim	0.049	0.922
Number of reflections	22033
<I/σ(I)>	12.1
Completeness [%]	99.5
Redundancy	6.8
CC(1/2)	0.997	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293.15	20% PEG3350, 0.2 M potassium fluoride pH 8.5, 0.1 M bicine