6O9Q
Wild-type SaSQS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 63.420, 85.840, 220.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.945 - 1.900 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uv0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.857 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.950 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.075 | 1.460 |
| Rpim | 0.023 | 0.457 |
| Number of reflections | 47868 | 3065 |
| <I/σ(I)> | 15.2 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 11 |
| CC(1/2) | 0.999 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294.15 | 6% (v/v) Tacsimate, 0.1 M MES monohydrate (pH = 6.0), 25% (w/v) polyethylene glycol 4000 |
