6O9N

Structural insights on a new fungal aryl-alcohol oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458
Spacegroup name	P 2 21 21
Unit cell lengths	63.854, 109.696, 220.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.192 - 2.598
R-factor	0.1965
Rwork	0.194
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fim
RMSD bond length	0.011
RMSD bond angle	1.257
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.192	2.680
High resolution limit [Å]	2.598	2.600
Number of reflections	48645	4296
<I/σ(I)>	7.3
Completeness [%]	99.7
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	0.1 M Tris-HCl, pH 9.0, 15% w/v PEG6000, 0.2 M calcium chloride