6O8H

Crystal structure of UvrB mutant bound to duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979200
Spacegroup name	P 41 21 2
Unit cell lengths	124.791, 124.791, 96.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 2.390
R-factor	0.2297
Rwork	0.227
R-free	0.27740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.049
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.390	2.400
Number of reflections	30412	2965
<I/σ(I)>	18.9
Completeness [%]	99.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium sulfate, 18-20% PEG3350, 2 mM ADP, 5 mM magnesium chloride