6O8F

Crystal structure of UvrB bound to duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979200
Spacegroup name	P 1 21 1
Unit cell lengths	58.157, 265.250, 68.315
Unit cell angles	90.00, 114.39, 90.00

Refinement procedure

Resolution	132.620 - 2.810
R-factor	0.2217
Rwork	0.220
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.178
Data reduction software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	132.620	2.910
High resolution limit [Å]	2.810	2.810
Number of reflections	45596	4507
<I/σ(I)>	9.1
Completeness [%]	99.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100 mM Bis-Tris, pH 6.5, 100 mM ammonium acetate, 17-19% w/v PEG8000