6O89
Anti-CD28xCD3 CODV Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-21 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.068 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.050, 73.390, 150.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.257 - 2.090 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 4DTG Fv PDB 1YJD Fv and PDB 4XGZ constant domain |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.250 | 51.250 | 2.140 |
| High resolution limit [Å] | 2.090 | 9.350 | 2.090 |
| Rmerge | 0.095 | 0.029 | 0.761 |
| Rmeas | 0.111 | 0.035 | 0.889 |
| Rpim | 0.057 | 0.019 | 0.455 |
| Total number of observations | 342800 | 3623 | 25200 |
| Number of reflections | 90445 | 1054 | 6626 |
| <I/σ(I)> | 10.9 | 34 | 2 |
| Completeness [%] | 99.9 | 97.8 | 99.8 |
| Redundancy | 3.8 | 3.4 | 3.8 |
| CC(1/2) | 0.997 | 0.997 | 0.642 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 22% PEG3350, 0.18M sodium phosphate monobasic |
