6O86

Crystal Structure of SeMet UDP-dependent glucosyltransferases (UGT) from Stevia rebaudiana in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	97.982, 97.982, 90.962
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.451 - 1.799
R-factor	0.1743
Rwork	0.173
R-free	0.19570
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.738
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.500	1.830
High resolution limit [Å]	1.799	1.800
Number of reflections	47033	2332
<I/σ(I)>	13
Completeness [%]	99.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	15% (w/v) PEG-4000, 20% 2-propanol (v/v), 100 mM sodium citrate tribasic dihydrate buffer pH 5.6