6O7Z
Trypanosoma cruzi EIF4E5 translation initiation factor in complex with cap-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9586 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 102.910, 102.910, 42.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.560 - 2.700 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 7339 | 1176 |
<I/σ(I)> | 24.1 | |
Completeness [%] | 100.0 | |
Redundancy | 20.3 | |
CC(1/2) | 1.000 | 0.544 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | 0,1M BisTris pH 6, 30% PEG3350, 0,2M Lithium Sulfate, 20% Glycerol |