6O78
Crystal structure of Csm1-Csm4 cassette in complex with pppApApA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 154.184, 154.184, 182.474 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.690 - 2.800 |
R-factor | 0.26144 |
Rwork | 0.257 |
R-free | 0.30417 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mua |
RMSD bond length | 0.002 |
RMSD bond angle | 1.278 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.800 | 2.800 |
Rpim | 0.025 | |
Number of reflections | 32053 | 3130 |
<I/σ(I)> | 31.9 | |
Completeness [%] | 100.0 | |
Redundancy | 37.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1 M phosphate-citrate pH 4.2, 5% PEG3000, 25% 1,2-propanediol, and 10% glycerol |