6O6X

Crystal structure of Csm6 W14A/E337A mutant in complex with cA4 by cocrystallization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	54.148, 115.745, 161.037
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.090 - 2.110
R-factor	0.20258
Rwork	0.200
R-free	0.24923
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.612
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	56521	5188
<I/σ(I)>	9.9
Completeness [%]	95.8
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	10% PEG 8K, 0.2 M NaCl, 0.1 M Na/K phosphate pH 6.2