6O6J
Crystal structure of the LjCASTOR gating ring in the Ca2+ and Na+ condition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.949360 |
| Spacegroup name | I 4 |
| Unit cell lengths | 125.234, 125.234, 81.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.600 - 1.600 |
| R-factor | 0.1665 |
| Rwork | 0.166 |
| R-free | 0.18820 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 1.201 | |
| Rmeas | 1.382 | |
| Rpim | 0.671 | |
| Number of reflections | 160841 | 7449 |
| <I/σ(I)> | 34.54 | 1.983 |
| Completeness [%] | 98.8 | 90.21 |
| Redundancy | 5 | 3.8 |
| CC(1/2) | 0.405 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 21% w/v PEG550 MME, 50 mM magnesium acetate, 100 mM Tris, pH 8.2 |
