6O62
Crystal structure of Sec4p, a Rab family GTPase from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-12-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.301, 38.082, 43.115 |
| Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
| Resolution | 24.040 - 1.880 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z0a |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.536 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.073 | 0.332 |
| Number of reflections | 11798 | 515 |
| <I/σ(I)> | 20.62 | 2.16 |
| Completeness [%] | 94.2 | 83.7 |
| Redundancy | 4.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | Ammonium phosphate dibasic 2.4M, Tris 0.1M pH 8.5 |
