6O5W

Crystal structure of MORC3 CW domain fused with viral influenza A NS1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-08-11
Detector	RDI CMOS_8M
Wavelength(s)	1.28
Spacegroup name	P 21 21 21
Unit cell lengths	30.912, 37.424, 50.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.338 - 1.412
R-factor	0.1544
Rwork	0.153
R-free	0.16870
Structure solution method	SAD
Starting model (for MR)	5svx
RMSD bond length	0.005
RMSD bond angle	0.948
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.430
High resolution limit [Å]	1.410	1.410
Number of reflections	71940	554
<I/σ(I)>	92.4
Completeness [%]	99.2
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	100 mM HEPES pH 7.5 and 1.4M Sodium Citrate