6O5M

Tubulin-RB3_SLD-TTL in complex with compound 10bb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	104.966, 157.721, 181.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.763 - 2.300
R-factor	0.1918
Rwork	0.190
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.109	0.061
Rmeas	0.113	0.064
Rpim	0.032	0.018	0.289
Number of reflections	135595	7184	6725
<I/σ(I)>	5.5
Completeness [%]	99.9	99.6	99.8
Redundancy	13.1	13.1	13
CC(1/2)		0.998	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2