6O43

Crystal structure of a lysin protein from Staphylococcus phage P68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-30
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.4590
Spacegroup name	P 31 2 1
Unit cell lengths	61.397, 61.397, 101.606
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.730 - 2.082
R-factor	0.182250142966
Rwork	0.179
R-free	0.22449
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.483
Data reduction software	XDS
Data scaling software	Aimless (v0.7.2)
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (dev_2689)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.730	2.130
High resolution limit [Å]	2.082	2.082
Rmeas	0.060	0.578
Rpim	0.013	0.134
Number of reflections	13689	853
<I/σ(I)>	22.2	3.5
Completeness [%]	98.3	80
Redundancy	19.5	17.1
CC(1/2)	1.000	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	289	0.04 M Citric acid, 0.06 M BIS-TRIS propane / pH 6.4, 20% w/v Polyethylene glycol 3,350