6O3X
Crystal structure of yeast Nrd1 CID in complex with Sen1 NIM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.690, 102.400, 115.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.345 - 1.994 |
R-factor | 0.1813 |
Rwork | 0.179 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3clj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.761 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 1.994 | 2.000 |
Rmerge | 0.080 | 0.487 |
Number of reflections | 39419 | |
<I/σ(I)> | 12.2 | |
Completeness [%] | 98.1 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium citrate, lithium chloride, PEG 6000 |